例句與造句

1. the recombinants were constructed by transforming ppic9 a-xynb into p . pastoris gs115 . the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p . pastoris . in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity . the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1 . xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd . we comparised the enzymatic properties of xynba expressed in p . pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5.2, the optimal ph of xynbb was 5.0; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ?; because of glycosylation the thermal stability of xynba was better than xynb and xynbb; the specific activity of xynba and xynbb were 883.88iu / mg and 832.5hu / mg respectively, which were both lower than 2814.45iu / mg of xynb; the km values of xynb and xynba were similar to each other which were 21.56 ( g / kg ) and 20.87 ( g / kg ), while the km value of xynbb was 27.10 ( g / kg ); the fmax of xynba and xynbb were 4568umol / mg . min and 5329umol / mg . min respectively which were lower than 27623 umol / mg . min of xynb; additionally all of the three enzymes did not display cellulase activity . they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent . the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68.43 % and 16.50 % respectively, additionally there was 11.79 % of xylobiose; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81.78 % and 11.55 % . the result indicated that this xylanase was a kind of 1, 4-b-d-xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
   進(jìn)一步對(duì)xynba進(jìn)行了脫糖基化處理得到xynbb,其分子量恢復(fù)到23kd，證明xynba是糖基化蛋白。通過(guò)對(duì)畢赤酵母重組表達(dá)的木聚糖酶xynba、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈霉菌a1所產(chǎn)原酶xynb之間酶學(xué)性質(zhì)的比較發(fā)現(xiàn)：三種酶的最適ph差異不大，xynb和xynba均為5.2，xynbb為5.0；xynb和xynba的最適溫度均為60，xynbb降為50：在耐熱性上，xynba由于糖基化作用熱穩(wěn)定性明顯高于未糖基化的xynb和xynbb；xynba和xynbb的比活性分別為883.88iumg和832.51iumg，明顯低于原酶的比活2814.45iumg；xynb和xynba的km值相當(dāng)，分別為21.56(gkg)和20.87(gkg)，而xynbb的km值較大為27.10(gkg)；xynba和xynbb的vmax相差不大，分別為4568molmg?min和5329molmg?min，明顯低于xynb的27623molmg?min此外三種酶均無(wú)纖維素酶活性，對(duì)胃蛋白酶和胰蛋白酶有很好的抗性，且對(duì)作用環(huán)境中的各種離子、表面活性劑、螯合劑不敏感。通過(guò)對(duì)不同木聚糖的酶解產(chǎn)物的糖份分析發(fā)現(xiàn)：以樺木木聚糖為底物時(shí)，酶解產(chǎn)物主要為木三糖和木四糖，含量分別為68.43和16.50，另外還含有11.79的木二糖；以玉米芯木聚糖為底物時(shí)，酶解產(chǎn)物主要為木二糖和木三糖，含量分別為81.78和11.55。
2. the recombinants were constructed by transforming ppic9 a-xynb into p . pastoris gs115 . the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p . pastoris . in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity . the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1 . xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd . we comparised the enzymatic properties of xynba expressed in p . pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5.2, the optimal ph of xynbb was 5.0; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ?; because of glycosylation the thermal stability of xynba was better than xynb and xynbb; the specific activity of xynba and xynbb were 883.88iu / mg and 832.5hu / mg respectively, which were both lower than 2814.45iu / mg of xynb; the km values of xynb and xynba were similar to each other which were 21.56 ( g / kg ) and 20.87 ( g / kg ), while the km value of xynbb was 27.10 ( g / kg ); the fmax of xynba and xynbb were 4568umol / mg . min and 5329umol / mg . min respectively which were lower than 27623 umol / mg . min of xynb; additionally all of the three enzymes did not display cellulase activity . they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent . the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68.43 % and 16.50 % respectively, additionally there was 11.79 % of xylobiose; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81.78 % and 11.55 % . the result indicated that this xylanase was a kind of 1, 4-b-d-xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
   進(jìn)一步對(duì)xynba進(jìn)行了脫糖基化處理得到xynbb,其分子量恢復(fù)到23kd，證明xynba是糖基化蛋白。通過(guò)對(duì)畢赤酵母重組表達(dá)的木聚糖酶xynba、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈霉菌a1所產(chǎn)原酶xynb之間酶學(xué)性質(zhì)的比較發(fā)現(xiàn)：三種酶的最適ph差異不大，xynb和xynba均為5.2，xynbb為5.0；xynb和xynba的最適溫度均為60，xynbb降為50：在耐熱性上，xynba由于糖基化作用熱穩(wěn)定性明顯高于未糖基化的xynb和xynbb；xynba和xynbb的比活性分別為883.88iumg和832.51iumg，明顯低于原酶的比活2814.45iumg；xynb和xynba的km值相當(dāng)，分別為21.56(gkg)和20.87(gkg)，而xynbb的km值較大為27.10(gkg)；xynba和xynbb的vmax相差不大，分別為4568molmg?min和5329molmg?min，明顯低于xynb的27623molmg?min此外三種酶均無(wú)纖維素酶活性，對(duì)胃蛋白酶和胰蛋白酶有很好的抗性，且對(duì)作用環(huán)境中的各種離子、表面活性劑、螯合劑不敏感。通過(guò)對(duì)不同木聚糖的酶解產(chǎn)物的糖份分析發(fā)現(xiàn)：以樺木木聚糖為底物時(shí)，酶解產(chǎn)物主要為木三糖和木四糖，含量分別為68.43和16.50，另外還含有11.79的木二糖；以玉米芯木聚糖為底物時(shí)，酶解產(chǎn)物主要為木二糖和木三糖，含量分別為81.78和11.55。
3. It's difficult to find km value in a sentence. 用km value造句挺難的